2020-12-02 REL 2019-04-10 PDBe PDBe 2019-06-12 2019-06-26 State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles Shayan R, Mitterer V, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Pertschy B, Plisson-Chastang C 6rbd 6rbe Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism. Nat Commun 10 2754 2754 2019 31227701 doi:10.1038/s41467-019-10678-z 2041-1723 emd_4792.map.gz Image stored as Reals 384 384 384 0 0 0 383 383 383 384 384 384 409.72803 409.72803 409.72803 90.0 90.0 90.0 X Y Z -0.1702075 0.5282937 0.0029767943 0.020167656 1
1.067 1.067 1.067 0.075 Yeast pre-40S particle, purified using Tsr1-TAP as bait, in mutant conditions (deletion of aa 68-78 of Rps20) Structural class 1 Local sharpening using LocScale 36 C1-S20DeltaLoop State 1 of yeast pre-40S particles purified with Tsr1-TAP as bait, in mutant conditions (deletion of aa 68-78 of Rps20) protein C1-S20DeltaLoop State 1 of yeast pre-40S particles purified with Tsr1-TAP as bait, in mutant conditions (deletion of aa 68-78 of Rps20) 1.2 Saccharomyces cerevisiae S288c false protein 40S ribosomal protein S27-A 0.008893391 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S30-A 0.007137541 Baker's yeast ATCC 204508 / S288c 1 false nucleic-acid 20S ribosomal RNA 0.58011425 Saccharomyces cerevisiae S288C false AUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAG UGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCU AGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUU UGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUC GAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAA CGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGA UACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUG GUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUG GGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGU GGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUG GAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCA UCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUU CAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGG GAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUC GCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGG AAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCG UUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAU UUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCC UUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUG UGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAG CUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGA UCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGU AACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUAAA RNA OTHER protein 40S ribosomal protein S0-A 0.028083416 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S1-A 0.028798467 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S2 0.027490826 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S4-A 0.02946933 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S6-A 0.027054486 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S7-A 0.021658209 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S8-A 0.022537803 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S9-A 0.022487893 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S11-A 0.017785934 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S13 0.017059945 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S21-A 0.009758829 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S22-A 0.014650062 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S23-A 0.016073896 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S24-A 0.015362848 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S28-A 0.007605847 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S29-A 0.006675723 Baker's yeast ATCC 204508 / S288c 1 false protein Pre-rRNA-processing protein PNO1 0.030380623 Baker's yeast ATCC 204508 / S288c 1 false protein Essential nuclear protein 1 0.055207422 Baker's yeast ATCC 204508 / S288c 1 false protein Ribosome biogenesis protein TSR1 0.090876539 Baker's yeast ATCC 204508 / S288c 1 false protein Serine/threonine-protein kinase RIO2 0.049192852 Baker's yeast ATCC 204508 / S288c 1 false protein Dimethyladenosine transferase 0.036003836 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S5 0.0250726 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S12 0.015488631 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S14-A 0.014562655 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S15 0.016031907 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S16-A 0.01587749 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S17-A 0.015820413 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S18-A 0.017071641 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S19-A 0.015942125 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S25-A 0.012067272 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S3 0.026542789 Baker's yeast ATCC 204508 / S288c 1 false protein 40S ribosomal protein S20 0.013929044 Baker's yeast ATCC 204508 / S288c 1 false ETHANE 95 293 LEICA EM GP
blot 1.7-1.9 seconds before plunging.
FIELD EMISSION GUN 32.4 BRIGHT FIELD 800.0 3000.0 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 6480 6FAI 6EML particle 7.5 singleParticle EXACT BACK PROJECTION RELION 3.47 FSC 0.143 CUT-OFF C1 54130