2015-09-09 REL 2015-05-08 PDBe PDBe 2015-06-17 2015-07-29 Structure of adenovirus light particle Ad5/FC31 L2 Condezo GN, Marabini R, Ayora S, Carazo JM, Chillon M, San Martin C icosahedral virus assembly, adenovirus, virus maturation Condezo GN, Marabini R, Ayora S, Carazo JM, Chillon M, San Martin C Structures of adenovirus incomplete particles clarify capsid architecture and show maturation changes of packaging protein L1 52/55k. J.VIROL. 89 9653 9664 2015 26178997 doi:10.1128/JVI.01453-15 emd_3003.map.gz Image stored as Reals 256 256 256 0 0 0 255 255 255 256 256 256 1075.2 1075.2 1075.2 90.0 90.0 90.0 X Y Z -1.81644154 4.99485445 0E-8 1.00000000 1
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4.2 4.2 4.2 1.4 Reconstruction of one type of light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31 1 light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31 icosahedral virus capsid 125 virus Human adenovirus 5 150 Human adenovirus 5 5 true false SEROTYPE VIRUS-LIKE PARTICLE Homo sapiens VERTEBRATES 950 25 ETHANE LEICA EM CPC FIELD EMISSION GUN 12 BRIGHT FIELD 500 4000 FLOOD BEAM FEI EAGLE (4k x 4k) 2.26 FEI TECNAI F20 11-NOV-2013 GATAN LIQUID NITROGEN 200 50000 889 14 cross-correlation Chimera rigid body REAL 3iYN particle 0.03 Quantifoil R2/4 300 mesh Cu/Rh glow discharged grids
20 mm Hepes, 150 mm NaCl
7.8 singleParticle projection matching Xmipp, Relion micrograph 12.3 FSC 0.143, gold-standard I 6743
CTF detection, phase flip, particle selection, extraction and normalization with Xmipp. 2D and 3D classification, 3D refinement and postprocessing with Relion.