2012-10-10 REL 2012-06-25 PDBe PDBe 2012-07-09 2012-09-19 EM map of an influenza H3 hemagglutatin in complex with C05 Fab Khayat R, Lee JH, Ekiert DC, Wilson IA, Ward AB Influenza A, flu, hemagglutanin, HA neutralizing antibody, C5 fab, C05 Ekiert DC, Kashyap AK, Steel J, Rubrum A, Bhabha G, Khayat R, Lee JH, Dillon MA, O'Neil RE, Faynboym AM, Horowitz M, Horowitz L, Ward AB, Palese P, Webby R, Lerner RA, Bhatt RR, Wilson IA Cross-neutralization of influenza A viruses mediated by a single antibody loop. NATURE 489 526 532 2012 22982990 doi:10.1038/nature11414 emd_2139.map.gz Image stored as Reals 100 100 100 -50 -50 -50 49 49 49 100 100 100 218.0 218.0 218.0 90.0 90.0 90.0 X Y Z -1.44750535 8.02569771 0E-8 1.00000000 1
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2.18 2.18 2.18 1.4 Negative stain reconstruction of flu HA3 in complex with C5 fab
emd_2139.png
2 Influenza HA3 in complex with C5 Fab One HA trimer binds to 3 fabs protein Influenza A/Perth/16/2009 hemagglutinin HA3 3 Trimer A/Perth/16/2009 Influenza A virus Flu true unidentified baculovirus pDCE198 protein C05 antibody fab fragment Monomer true 3 Homo sapiens Human pFastBacDual unidentified baculovirus NONE NONE Objective lens astigmatism corrected at 100,000x FIELD EMISSION GUN 16 BRIGHT FIELD 700 293 900 FLOOD BEAM 293
Images taken from 0 to 55 degrees in 5 degree tilt increments
GENERIC GATAN (4k x 4k) 0 55 FEI TECNAI F20 14-FEB-2012 SIDE ENTRY, EUCENTRIC 120 100000 269 0.109 16
Images recorded using CCD
 Nano-W for 30 seconds 0.1 400 mesh copper grid particle
20mM HEPES pH 7.5, 50mM NaCl
7.5 singleParticle Sparx Micrograph and each particle 17 FSC 0.5 28766
Particles were selected using an automated particle picking program.
C3