2012-12-26
REL
2009-11-18
PDBe
PDBe
2010-01-26
2010-03-10
CryoEM Model of the Vesicular Stomatitis Virus
Ge P, Tsao J, Green TJ, Luo M, Zhou ZH
NSRV, VSV, CryoEM, RNA, helix
Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH
Cryo-EM model of the bullet-shaped vesicular stomatitis virus.
SCIENCE
327
689
693
2010
20133572
doi:10.1126/science.1181766
emd_1663_msk.map
Image stored as Reals
108
112
120
-196
-65
-31
-89
46
88
112
108
120
|
171.584
165.456
183.84
90.0
90.0
90.0
|
X
Y
Z
-2.970071
5.98934
0.11676611
0.6069033
1
::::EMDATABANK.org::::EMD-1663::::MASK:1::::
1.532
1.532
1.532
This is 2N with 5M in closest neighborhood of a central M-hub domain
emd_1663_msk_1.map
Image stored as Reals
164
128
84
-179
-153
-11
-16
-26
72
128
164
84
196.096
251.24802
128.688
90.0
90.0
90.0
|
X
Y
Z
-3.455964
7.0695634
0.08473596
0.5966924
1
::::EMDATABANK.org::::EMD-1663::::MASK:2::::
1.532
1.532
1.532
This is 5 consequetive N's in the nucleocapsid
1
VSV (Indiana)
Mature virion
virus
Vesicular stomatitis virus
VSV
Horse is the primary host species of the virus but Human is also possible.
Vesicular stomatitis virus
VSV
false
true
STRAIN
VIRION
Equus caballus
VERTEBRATES
manual blot 1 second before plunging
ETHANE
Vitrification instrument: manual plunger. Vitrification carried out in open room
50
HOMEMADE PLUNGER
80
Objective lens astigmatism was corrected at 250,000 times magnification
FIELD EMISSION GUN
20
BRIGHT FIELD
800
2500
SPOT SCAN
Eucentric
GENERIC TVIPS
2
98000
80
FEI POLARA 300
05-JAN-2007
SIDE ENTRY, EUCENTRIC
300
59000
212
1.532
16
Protocol: Rigid Body
rigid body
REAL
Cross-correlation
Chimera
2GIC
REAL
Cross-correlation
rigid body
Protocol: Rigid Body
Chimera
2W2R
LEFT HANDED
Cryo sample
Quantifoil 3.5/1 200 mesh
filament
7.4
100mM NaCl, 10nM Tris, 1mM EDTA
helical
projection matching
EMAN
Helicity determined and applied by IHRSR
10.6
FSC 0.5