2017-06-21 REL 2017-01-19 RCSB RCSB 2017-02-15 2017-05-31 CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 4 Liu Y, Pan J 5uk2 Liu Y, Pan J, Jenni S, Raymond DD, Caradonna T, Do KT, Schmidt AG, Harrison SC, Grigorieff N CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface. J. Mol. Biol. 429 1829 1839 2017 28506635 doi:10.1016/j.jmb.2017.05.011 1089-8638 0070 JMOBAK emd_8564.map.gz Image stored as Reals 120 120 120 -60 -60 -60 59 59 59 120 120 120 196.8 196.8 196.8 90.0 90.0 90.0 X Y Z -3.741372 8.258274999999999 0.071280874 0.45758796 1
1.64 1.64 1.64 2.52 influenza virus receptor-binding site antibody-antigen interface - Class 4 6 Influenza-virus hemagglutinin H1 complexed with K1915 single-chain variable-domain fragment 0.32
The complex consists of three hemagglutinin head domains bound to three single-chain variable-domain fragments.
protein Influenza-virus hemagglutinin H1 complexed with K1915 single-chain variable-domain fragment 0.32 Influenza A virus (A/Solomon Islands/3/2006(H1N1)) false Trichoplusia ni Hi-5
The complex consists of three hemagglutinin head domains bound to three single-chain variable-domain fragments.
 protein Hemagglutinin HA1 0.036024344 false Influenza A virus (A/Solomon Islands/3/2006(H1N1)) A/Solomon Islands/3/2006(H1N1) pFastBac protein Hemagglutinin HA2 0.019841041 false Influenza A virus A/Solomon Islands/3/2006(H1N1) pFASTBAC protein scFv 0.027629385 false Homo sapiens pVRC ligand BETA-D-MANNOSE 0.000180156 3 false ligand N-ACETYL-D-GLUCOSAMINE 0.000221208 30 false ETHANE 90.0 298.0 FEI VITROBOT MARK I FIELD EMISSION GUN 40.0 BRIGHT FIELD FLOOD BEAM GATAN K2 (4k x 4k) 2.7 30444 70.0 70.0 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 18000 10281 2.5
The exposure rate was 8 electrons/physical pixel/second.
 particle 0.1 8.
Beta-octylglucoside was added to a final concentration of 0.07% w/v to induce more variable particle orientations.
 singleParticle FOURIER SPACE Frealign 4.8 FSC 0.143 CUT-OFF
Refinement and classification were limited to 10 Angstrom resolution.
 142314
The particle images were normalized to have constant variance and zero average. Movies were processed using Unblur.