2013-08-21 REL 2013-06-26 RCSB RCSB 2013-07-03 2013-07-24 Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664 in complex with soluble CD4 Khayat R, Lee JH, Wilson IA, Ward AB Human immunodeficiency virus, antigen, vaccine development, neutralization, conformational change, micelles Klasse PJ, Depetris RS, Pejchal R, Julien JP, Khayat R, Lee JH, Marozsan AJ, Cupo A, Cocco N, Korzun J, Yasmeen A, Ward AB, Wilson IA, Sanders RW, Moore JP Influences on trimerization and aggregation of soluble, cleaved HIV-1 SOSIP envelope glycoprotein. J.VIROL. 87 9873 9885 2013 23824824 doi:10.1128/JVI.01226-13 emd_5708.map.gz Image stored as Reals 144 144 144 0 0 0 143 143 143 144 144 144 313.92 313.92 313.92 90.0 90.0 90.0 X Y Z -8.47443008 14.07864761 0.05913661 0.99994820 1
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2.18 2.18 2.18 4.29 Negative stained image reconstruction of HIV KNH11444 subtype A SOSIP.664 in complex with soluble CD4
emd_5708.png
2 HIV KNH11444 subtype A SOSIP.664 in complex with soluble CD4 Trimer 0.538
The sample was monodisperse via SEC.
protein Human immunodeficiency virus Envelope protein gp120/gp41 0.478 3 Trimer KNH1144 Human immunodeficiency virus HIV true Homo sapiens 293S pPI4 protein CD4 false Homo sapiens human NONE NONE Objective lens astigmatism was corrected at 100,000 times magnification FIELD EMISSION GUN 16 BRIGHT FIELD 300 293 900 FLOOD BEAM 294 GENERIC GATAN (4k x 4k) 2 0 100000 55 293 FEI TECNAI F20 25-AUG-2012 -2 SIDE ENTRY, EUCENTRIC 120 100000 220 10.9 16
Data collected on CCD
 2% Uranyl Formate for 30 seconds particle 0.1 400 mesh copper grid
20 mM HEPES, pH 7.5, 50 mM NaCl
7.5 singleParticle Cross-common lines SPIDER: theta 45 degrees, phi 45 degrees Sparx/EMAN2 Each particle
Final maps were calculated from two averaged data sets.
16 FSC 0.5 512 C3 16704
Particles were selected using DogPicker, reference free class averages determined using Sparx, and initial model generated using common-lines with EMAN2.