2011-09-06 REL 2011-02-16 RCSB RCSB 2011-03-10 2011-03-10 Seeing the Portal in Herpes Simplex Virus type I B-capsids. Rochat RH, Liu X, Murata K, Nagayama K, Rixon FJ, Chiu W Herpes, HSV1, Portal, Cryo-EM, Single Particle, UL6, Asymmetric, Symmetry-free Rochat RH, Liu X, Murata K, Nagayama K, Rixon FJ, Chiu W Seeing the portal in herpes simplex virus type 1 B capsids. J.VIROL. 85 1871 1874 2011 21106752 doi:10.1128/JVI.01663-10 emd_5259.map.gz Image stored as Reals 300 300 300 -150 -150 -150 149 149 149 300 300 300 1596 1596 1596 90 90 90 X Y Z -7.80531 6.50117 0.327607 1.45926 1
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5.32 5.32 5.32 2.4 Icosahedral reconstruction of the HSV-1 B-capsid 1 Herpes simplex virus type I B-capsids Icosahedral 100 virus Herpes Simplex Virus Type I HSV-1 200 Herpes Simplex Virus Type I HSV-1 false false OTHER STRAIN VERTEBRATES Homo sapiens vp5 16 1250 1250 15 vp26 10 vp19 1250 1250 vp23 5 Blot 1 time for 2 seconds ETHANE
Vitrification instrument: Vitrobot Mark IV
100 FEI VITROBOT MARK IV 85 Astigmatism was corrected at 400,000 times magnification FIELD EMISSION GUN 20 Omega BRIGHT FIELD 0 100 200 FLOOD BEAM 101 Side Entry GENERIC TVIPS (4k x 4k) 4.1 0 56000 0 100 JEOL 2200FS 10-JUN-2009 GATAN LIQUID NITROGEN 200 40000 0-20 Quantifoil 2/2 grids particle singleParticle Cross-common lines MPSA No Correction
Reconstructions were made both with and without imposed symmetry.
24 FSC 0.5
The particles were aligned using the multi-path simulated annealing algorithm.
6300 I