2014-11-26 REL 2011-06-10 PDBe PDBe 2011-07-06 2011-07-06 Unique structure of iC3b by 3D-Electron Microscopy Alcorlo M, Martinez-Barricarte R, Fernandez FJ, Rodriguez-Gallego C, Round A, Vega MC, Harris CL, Rodriguez-de-Cordoba S, Llorca O iC3b, C3b, complement, 3D-electron microscopy, SAXS Alcorlo M, Martinez Barricarte R, Fernandez FJ, Rodriguez-Gallego C, Round A, Vega MC, Harris CL, Rodriguez de Cordoba S, Llorca O Unique structure of iC3b resolved at 24 A resolution by 3D-Electron Microscopy PROC.NAT.ACAD.SCI.USA 108 13236 13240 2011 21788512 doi:10.1073/pnas.1106746108 emd_1908.map.gz Image stored as Reals 72 72 72 -9 -9 -9 62 62 62 72 72 72 302.4 302.4 302.4 90.0 90.0 90.0 X Y Z -13.07929325 13.85720444 0E-8 0.97025698 1
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4.2 4.2 4.2 4.59 3D structure of iC3b, a component of the complement alternative pathway 1 Human iC3b Monomer 0.181 protein Complement component iC3b iC3b 0.181 Homo sapiens false Monomer Human 1 Plasma NONE NONE Objective lens astigmatism was corrected at 80,000 times magnification TUNGSTEN HAIRPIN 10 BRIGHT FIELD 1000 1500 FLOOD BEAM Eucentric KODAK SO-163 FILM 2.9 0 47 JEOL 1230 JEOL 100 50000 OTHER 112 10.5 16
Images scanned with Minolta Dimage Scan Multi Pro scanner at 2400 dpi and averaged to a final 4.2 Angstroms pixel at the specimen
 Cross-correlation
Protocol: Rigid Body
Situs rigid body REAL 2I07 1% w/v uranyl acetate 1.1 Carbon-coated 400 mesh copper grid particle
150 mM NaCl, 20 mM Tris-HCL
7.4 singleParticle Angular refinement using as initial template a random conical tilt model Xmipp,EMAN 24 FSC 0.5 29730
The particles were selected manually with Boxer (Eman)
C1