2014-04-16 REL 2006-07-10 PDBe PDBe 2006-07-10 2007-05-02 Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. Luque D, Saugar I, Rodriguez JF, Verdaguer N, Garriga D, San Martin C, Velazquez-Muriel JA, Trus BL, Carrascosa JL, Caston JR Luque D, Saugar I, Rodriguez JF, Verdaguer N, Garriga D, Martin CS, Velazquez-Muriel JA, Trus BL, Carrascosa JL, Caston JR Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J.VIROL. 81 6869 6878 2007 17442720 doi:10.1128/JVI.00077-07 emd_1237.map.gz Image stored as Reals 243 243 243 -121 -121 -121 121 121 121 243 243 243 340.2 340.2 340.2 90 90 90 X Y Z -2.20102 3.09582 0.00422405 0.263223 1
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1.4 1.4 1.4 0.338 Density map of the T=1 Infectious Bursal Disease Virus (IBDV) Subviral Particle (SVP)2X2Y2Z oriented. Infectious Bursal Disease Virus Subviral Particle 60 copies of VP2 arranged in 20 trimers 1 2.8 virus Infectious bursal disease virus Gumboro virus, subviral particle 2.880
IBDV Capsid Protein
Infectious bursal disease virus Gumboro virus, subviral particle true false STRAIN VIRUS-LIKE PARTICLE Gallus gallus VERTEBRATES 260 IBDV Subviral particle 1 ETHANE FEI TECNAI F20 Eucentric GATAN LIQUID NITROGEN 200 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 23 7 8 ZEISS SCAI
Protocol: Rigid body. Refinement was done in both Real and Fourier Sapce
rigid body REAL CC and R-factor SITUS and URO 1WCD REAL CC and R-factor rigid body
Protocol: Rigid body. Refinement was done in both Real and Fourier Sapce
SITUS and URO 2gsy Samples containing were applied to one side of a holey carbon film, washed twice on water drops, blotted, and plunged into a liquid ethane bath following standard procedures 300 mesh holey carbon film particle
25 mM PIPES, 150 mM NaCl, and 20 mM CaCl2
6.2 singleParticle Common lines em3dr2 Each particle 7.2 FSC 0.333
Resolution for SVP was also evaluated by FSC calculated between the full-dataset map and the SVP atomic map and for a correlation limit of 0.3, this was at 6.6 A.
 23754 I