2012-10-17 REL 2006-07-10 2006-07-10 2007-05-02 Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. D. Luque, I.Saugar, J.F. Rodriguez, N. Verdaguer, D. Garriga, C. San Martin, J.A. Velazquez-Muriel, B.L. Trus, J.L. Carrascosa, J.R. Caston 2gsy Luque D, Saugar I, Rodriguez JF, Verdaguer N, Garriga D, Martin CS, Velazquez-Muriel JA, Trus BL, Carrascosa JL, Caston JR Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch. J.VIROL. 81 6869 6878 2007 17442720 doi:10.1128/JVI.00077-07 EMD-1237.map float (32-bit) 243 243 243 -121 -121 -121 121 121 121 243 243 243 340.2 340.2 340.2 90 90 90 X Y Z -2.20102 3.09582 0.00422405 0.263223 1
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1.4 1.4 1.4 0.338 Density map of the T=1 Infectious Bursal Disease Virus (IBDV) Subviral Particle (SVP)2X2Y2Z oriented. icosahedral Infectious Bursal Disease Virus Subviral Particle 60 copies of VP2 arranged in 20 trimers 1 2.8 virus Infectious bursal disease virus Gumboro virus, subviral particle 2880
IBDV Capsid Protein
Infectious bursal disease virus Gumboro virus, subviral particle Gallus gallus VERTEBRATES 00.009.0.02.001 true false STRAIN VIRUS-LIKE PARTICLE 260 IBDV Subviral particle 1 ETHANE FEI TECNAI F20 Eucentric GATAN LIQUID NITROGEN 200 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 23 7 8 ZEISS SCAI Rigid body CC and R-factor SITUS and URO
Refinement was done in both Real and Fourier Sapce
1WCD
25 mM PIPES, 150 mM NaCl, and 20 mM CaCl2
6.2 Samples containing were applied to one side of a holey carbon film, washed twice on water drops, blotted, and plunged into a liquid ethane bath following standard procedures 300 mesh holey carbon film Common lines em3dr2 Each particle 7.2 FSC at 0.3 cut-off
Resolution for SVP was also evaluated by FSC calculated between the full-dataset map and the SVP atomic map and for a correlation limit of 0.3, this was at 6.6 A.
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